Journal: Nature Communications

Article Title: A covalent peptide-based lysosome-targeting protein degradation platform for cancer immunotherapy

doi: 10.1038/s41467-025-56648-6

Figure Lengend Snippet: a The endocytosis of 25 μM Biotin-v9x in B16-sh-NT and B16-sh-mTFRC cells (incubated at 37 °C for 2 h) by confocal imaging, scale bar: 20 μm. n = 3. Data are expressed as means ± SEM. b The dynamic colocalization of PD-L1 (red) with lysosomes (Lamp1, green) and TFRC (purple) in B16 cells incubated with 25 μM v9x for 0, 2, 4 or 6 h, as shown by confocal images, scale bar: 5 μm. The white dashed box indicates the magnified region, and the fluorescence gray level plot on the right is derived from the solid white box region. c The affinity between PD-1-Fc protein (100 ng) and PD-L1 on the surface of 293T-mPD-L1 (TFRC low expression), B16, and MC38 cells with 25 μM covalent Pep-TACs for 48 h. n = 3. Data are expressed as means ± SEM. Three independent experiments were performed with similar results and representative results are shown. Source data are provided as a Source Data file. All experiments were analyzed by unpaired two-tailed t -tests.

Article Snippet: After sealing with 5% non-fat milk ( >1h at RT), the membranes were incubated with primary antibodies for mPD-L1 (D363307, BBI, used at 1/500 dilution; GB12339, Servicebio, used at 1/500 dilution), GAPDH (D110016, BBI, used at 1/5000 dilution), β-actin (T0022, Affinity, used at 1/5000 dilution) overnight at 4 °C.

Techniques: Incubation, Imaging, Fluorescence, Derivative Assay, Expressing, Two Tailed Test